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In Situ Hybridization Protocols (Methods in Molecular Biology)
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Home > Medical Books > Colloidal Silver > Item 4
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In Situ Hybridization Protocols (Methods in Molecular Biology)
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by Ian A. Darand Tim D. Hewitson
Sales Rank: 331708
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$104.00
At Amazon
on 10-24-2008
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Hardcover: 280 pages
Publisher: Humana Press; 3 edition December 15, 2005
Language: English
ISBN-10: 1588294021
ISBN-13: 978-1588294029
Product Dimensions:
9.2 x 5.9 x 0.9 inches
Shipping Weight: 1.6 pounds
Product Review
Reviews of Previous Editions:
"an indispensable laboratory manual for the current techniques usedessential for the current techniques usedessential for any laboratory that is using, or wants to use, thetechnique in their repertoire."-Molecular Biotechnology
" . . .a good book for anyone wishing to exploit all that in situ hybridization has to offer."
-- Biopharm Manufacturing
Reviews for Second Edition
"This book should be of interest to anyone using or considering in situ hybridization, as it provides well written, if rather specialized, examples of the many applications of this extremely useful but notoriously tricky techniqueThe text is well referenced and includes useful details of reagent suppliers as well as links to further information available over the internet. This is a book that aims to make in situ hybridization accessible to all, and to inspire new applications for the technique. In my opinion, it does a reasonably sound job of both."-Heredity
From Reviews of the Second Edition
"highly recommended for those researchers who use in situ hybridization in their work or for those who want to exploit for the first time the possibilities that this technique offers biomedical research" -European Urology Today
"should be available in every major biomedical library as it represents the approach that morphological scientists can use to determine gene expression under different physiological and disease conditions in mature and developing tissues." -Journal of Anatomy
From the reviews of the third edition:
"In situ hybridization is already 25 years known method, which allows to detect specific RNA and DNA sequences at the cellular level within tissue sections. The third edition of this book focuses on tissue and cell in situ hybridization. The book is written with a broad readership in mind. It provides information for all researchers to set up this technique in the laboratory, as well as to update the recent protocols, which are already in use." (H. Plchová, Biologia Plantarum, Vol. 50 (4), 2006)
"Darby and Hewitson have extensively changed the content and in doing so have made the book much more relevant and useful to the veterinary pathologist. The new third edition focuses on hybridization of cells and tissues, instead of having a major focus on chromosomes as in the previous edition. reading the book cover-to-cover allows one to gain a collective appreciation for various alternatives in methodology as well as interesting innovative procedural details." (Dr. R. A. Westhouse, Veterinary Pathology, Vol. 43 (4), 2006)
Product Description
This revised and updated edition of a recognized classic emphasizes tissue and cell in situ hybridization methods. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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In Situ Hybridization Protocols (Methods in Molecular Biology)
Available from Amazon
Price: $104.00
Updated on 10-24-2008
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